Ultraviolet (UV) irradiation is considered to be the primary environmental factor that causes skin damage. In the present study, we investigated the protective effect of dieckol (DK), a compound isolated from the brown seaweed Ecklonia cava, against UVB-induced skin damage in human dermal fibroblasts (HDF cells). The results indicated that DK effectively inhibited the activity of collagenase. DK remarkably reduced the intracellular reactive oxygen species level and improved the viability of UVB-irradiated HDF cells. Besides, DK significantly and dose-dependently improved collagen synthesis and inhibited intracellular collagenase activity in UVB-irradiated HDF cells. In addition, DK markedly reduced the expression of proinflammatory cytokines and matrix metalloproteinases. Further analyses revealed that these processes were mediated through the regulation of nuclear factor kappa B, activator protein 1, and mitogen-activated protein kinase signaling pathways in the UVB-irradiated HDF cells. In conclusion, these results indicate that DK possesses strong in vitro photoprotective effects and therefore has the potential to be used as an ingredient in the cosmeceutical industry.
Le radiazioni UV sono i principali fattori ambientali responsabili del danneggiamento cutaneo. L'obbiettivo di questo studio è testare l'azione foto-protettiva del Dieckol, principale florotannino dell'alga bruna Ecklonia Cava, contro il foto-invecchiamento cutaneo indotto dai raggi UV nei fibroblasti dermici umani. L'analisi dei meccanismi molecolari rivelano le modalità di azione del Dieckol, che funge da: scavenger di ROS con conseguente aumento della vitalità cellulare, regolatore di diversi pathways metabolici come NF-kB, MAPKs e AP-1 con conseguente attenuazione dell'espressione di citochine pro-infiammatorie e MMPs, inibitore dell'attività della collagenasi intracellulare e attivatore della sintesi di collagene, soppressa dai raggi UV. I risultati ottenuti determinano un elevato potere anti-ossidante, anti-infiammatorio e anti-apoptico del Dieckol in vitro, suggerendo una sua applicazione in vivo all'interno di formulazioni farmaceutiche/cosmetiche, al fine di migliorare e posticipare il processo di foto-invecchiamento cutaneo.
Dieckol e la sua azione foto-protettiva contro il danneggiamento cutaneo indotto dai raggi UV.
BONCI, NICOLETTA
2021/2022
Abstract
Ultraviolet (UV) irradiation is considered to be the primary environmental factor that causes skin damage. In the present study, we investigated the protective effect of dieckol (DK), a compound isolated from the brown seaweed Ecklonia cava, against UVB-induced skin damage in human dermal fibroblasts (HDF cells). The results indicated that DK effectively inhibited the activity of collagenase. DK remarkably reduced the intracellular reactive oxygen species level and improved the viability of UVB-irradiated HDF cells. Besides, DK significantly and dose-dependently improved collagen synthesis and inhibited intracellular collagenase activity in UVB-irradiated HDF cells. In addition, DK markedly reduced the expression of proinflammatory cytokines and matrix metalloproteinases. Further analyses revealed that these processes were mediated through the regulation of nuclear factor kappa B, activator protein 1, and mitogen-activated protein kinase signaling pathways in the UVB-irradiated HDF cells. In conclusion, these results indicate that DK possesses strong in vitro photoprotective effects and therefore has the potential to be used as an ingredient in the cosmeceutical industry.| File | Dimensione | Formato | |
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https://hdl.handle.net/20.500.12075/12405