Cephalopods have unique cognitive abilities and behaviors. Some life and behavioral strategies are not yet fully understood. For example, coastal octopuses have been documented to occasionally leave the water and crawl into intertidal areas, but there is a complete lack of knowledge about the physiological and biochemical basis of this behavior. This study aimed to investigate, for the first time, the physiological and biochemical responses (activities of antioxidant enzymes, levels of heat shock proteins and ubiquitin, DNA damage, lipid peroxidation) of the common octopus, Octopus vulgaris, following emergence. The physiological performance of the octopuses was determined by measuring metabolic rates in different emergence treatments and biochemical markers. Size-adjusted maximum metabolic rates (MMRadj) of octopuses exposed to 2:30 min of air exposure followed by re-immersion did not differ significantly from the MMRadj of chased individuals (control group). However, most biochemical markers did not reveal significant differences between the different surfacing treatments.The results showed that Octopus vulgaris can tolerate exposure to short-term surfacing periods thanks to an efficient antioxidant system and cellular repair mechanisms. It is argued that the use of atmospheric air through mucus-coated gills and/or cutaneous breathing allows the octopus to resist surfacing and crawling on land.
I cefalopodi presentano capacità cognitive e comportamenti unici. Alcune strategie vitali e comportamentali non sono ancora stati del tutto compresi. Ad esempio, è stato documentato che i polpi costieri occasionalmente lasciano l'acqua e strisciano nelle aree intertidali, ma c'è una totale mancanza di conoscenza sulla base fisiologica e biochimica di questo comportamento. Questo studio ha lo scopo di indagare, per la prima volta, le risposte fisiologiche e biochimiche (attività degli enzimi antiossidanti, livelli di proteine da shock termico e ubiquitina, danni al DNA, perossidazione lipidica) del polpo comune, Octopus vulgaris, in seguito all'emersione. Le prestazioni fisiologiche dei polpi sono state determinate misurando i tassi metabolici in diversi trattamenti di emersione e marcatori biochimici. I tassi metabolici massimi aggiustati per dimensione (MMRadj) dei polpi esposti a 2:30 min di esposizione all'aria seguiti da reimmersione non differivano in modo significativo dai MMRadj degli individui inseguiti (gruppo di controllo). Tuttavia, la maggior parte dei marcatori biochimici non ha rivelato differenze significative tra i diversi trattamenti di emersione. I risultati hanno mostrato che Octopus vulgaris può tollerare l'esposizione a periodi di emersione di breve durata grazie a un efficiente sistema antiossidante e a meccanismi di riparazione cellulare. Si sostiene che l'uso di aria atmosferica attraverso le branchie ricoperte di muco e/o la respirazione cutanea permetta al polpo di resistere all'emersione e allo strisciamento sulla terraferma.
Risposte fisiologiche e biochimiche di Octopus vulgaris all’emersione
NANNI, MICAELA
2023/2024
Abstract
Cephalopods have unique cognitive abilities and behaviors. Some life and behavioral strategies are not yet fully understood. For example, coastal octopuses have been documented to occasionally leave the water and crawl into intertidal areas, but there is a complete lack of knowledge about the physiological and biochemical basis of this behavior. This study aimed to investigate, for the first time, the physiological and biochemical responses (activities of antioxidant enzymes, levels of heat shock proteins and ubiquitin, DNA damage, lipid peroxidation) of the common octopus, Octopus vulgaris, following emergence. The physiological performance of the octopuses was determined by measuring metabolic rates in different emergence treatments and biochemical markers. Size-adjusted maximum metabolic rates (MMRadj) of octopuses exposed to 2:30 min of air exposure followed by re-immersion did not differ significantly from the MMRadj of chased individuals (control group). However, most biochemical markers did not reveal significant differences between the different surfacing treatments.The results showed that Octopus vulgaris can tolerate exposure to short-term surfacing periods thanks to an efficient antioxidant system and cellular repair mechanisms. It is argued that the use of atmospheric air through mucus-coated gills and/or cutaneous breathing allows the octopus to resist surfacing and crawling on land.File | Dimensione | Formato | |
---|---|---|---|
Presentazione Micaela Nanni_SP.pdf
accesso aperto
Dimensione
739.93 kB
Formato
Adobe PDF
|
739.93 kB | Adobe PDF | Visualizza/Apri |
I documenti in UNITESI sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/20.500.12075/20500