Recent studies show that dietary probiotics supplementation can have beneficcial effects on both humans and animals. I demonstrated with the poriobiotic lactobacillus rhamnosus - a compponent of the human gut microflora, which enhances reproduction, larval development, and the biomineralization process in Danio rerio (commonly known as zebrafish). The aim of this study was to identify the pathways affected by L. rhamnosus during zebrafish larval development. Our morphological and histochemical findings show that L. rhamnosus accelerates bone deposition through stimulation of the expression of the key genes involved in ossification. eg: runt-related transcription factor 2(runx2), Sp7 transcription factor(sp7), matrix Gla protein (mgp), and bone gamma-carboxyglutamate(gla) protein (bglap) as well as through inhibition of sclerostin(sost) - a bone formation inhibitor. Western blot analysis of mitogen-activated protein kinase 1 and 3 (Mapk1 and Mapk3), which are involved in osteoblast and osteocyte differentiation showed an increase in Mapk1 16 days post fertilization (dpf) in individuals receiving L. rhamnosus supplementation. In my studies, I found out that a reduction of sclerostin(sost) that is detected in individuals receiving L. rhamnosus suggests that the probiotic may help treat bone disorder. Through molecular and histological techniques such as double staining, I was ableto identify changes that happen during larval development due to probiotic supplementation. Other gene expression changes were identified after real-time PCR precess.

Il probiotico promuove il processo di calcificazione in larve di Danio rerio: uno studio molecolare

GYAMFI, PRINCE
2019/2020

Abstract

Recent studies show that dietary probiotics supplementation can have beneficcial effects on both humans and animals. I demonstrated with the poriobiotic lactobacillus rhamnosus - a compponent of the human gut microflora, which enhances reproduction, larval development, and the biomineralization process in Danio rerio (commonly known as zebrafish). The aim of this study was to identify the pathways affected by L. rhamnosus during zebrafish larval development. Our morphological and histochemical findings show that L. rhamnosus accelerates bone deposition through stimulation of the expression of the key genes involved in ossification. eg: runt-related transcription factor 2(runx2), Sp7 transcription factor(sp7), matrix Gla protein (mgp), and bone gamma-carboxyglutamate(gla) protein (bglap) as well as through inhibition of sclerostin(sost) - a bone formation inhibitor. Western blot analysis of mitogen-activated protein kinase 1 and 3 (Mapk1 and Mapk3), which are involved in osteoblast and osteocyte differentiation showed an increase in Mapk1 16 days post fertilization (dpf) in individuals receiving L. rhamnosus supplementation. In my studies, I found out that a reduction of sclerostin(sost) that is detected in individuals receiving L. rhamnosus suggests that the probiotic may help treat bone disorder. Through molecular and histological techniques such as double staining, I was ableto identify changes that happen during larval development due to probiotic supplementation. Other gene expression changes were identified after real-time PCR precess.
2019
2020-12-19
Probiotic Supplementation Promotes Calcification in Danio rerio Larvae: a Molecular Study
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12075/3351