The gilthead seabream, Sparus aurata, is one of the main reared species in the Mediterranean region, and therefore it is subjected to the negative consequences of intensive aquaculture practice such as the occurrence of infectious disease outbreaks. The administration of postbiotics to farmed species represented a sustainable and safe practice with well-known immune-busting properties. This study investigated the secondary effects of butyric acid (commonly used as postbiotic), encapsulated in a starch-based capsule, on sea bream lipid metabolism. Juvenile gilthead seabreams were divided into three experimental groups and fed for 12 weeks three experimental diets: a control group (CTRL) of fish fed the commercial diet, a POP group of fish fed the same commercial diet of the control group added with empty microcapsules and a POP+POST group of fish fed the same commercial diet of the control group added with butyric acid microencapsulated. Analysis of growth parameters (final body weight, FCR, SGR, and daily feed intake) revealed no significant differences among fish from the three experimental groups. Molecular analysis revealed a possible effect of the starch capsule on lipid metabolism. Differently, the administration of POP+POST did not alter the molecular pathways involved in lipid metabolism if compared to the control group.
L'orata, Sparus aurata, è la principale specie allevata in Mediterraneo, di conseguenza è soggetta alle conseguenze negative delle pratiche di acquacoltura intensive, come l’insorgenza di frequenti focolai di malattie infettive. L'amministrazione di postbiotici a specie allevate rappresenta una pratica sostenibile e sicura con proprietà ben note per il potenziamento del sistema immunitario. Questo studio ha indagato gli effetti secondari dell'acido butirrico (comunemente usato come postbiotico), incapsulato in una capsula a base di amido, sul metabolismo lipidico di Sparus aurata. I giovanili di orata sono stati suddivisi in tre gruppi sperimentali e alimentati per 12 settimane con tre diete sperimentali: un gruppo di controllo (CTRL) alimentati con una dieta commerciale, un gruppo POP alimentati con la stessa dieta commerciale del gruppo di controllo, ma con l’aggiunta di microcapsule vuote, e un gruppo POP+POST alimentati con la stessa dieta commerciale del gruppo di controllo, ma con l’aggiunta di microcapsule contenenti acido butirrico. L'analisi dei parametri di crescita (peso corporeo finale, FCR, SGR e assunzione giornaliera di mangime) non ha rivelato differenze significative tra i pesci dei tre gruppi sperimentali. Le analisi molecolari hanno evidenziato un possibile effetto della capsula a base di amido sul metabolismo lipidico. Al contrario, l'amministrazione di POP+POST non ha alterato le vie molecolari coinvolte nel metabolismo lipidico rispetto al gruppo di controllo.
Applicazione di postbiotici microincapsulati nell'allevamento di orata (Sparus aurata): focus sul metabolismo dei lipidi
HMIDA, SABRIN
2023/2024
Abstract
The gilthead seabream, Sparus aurata, is one of the main reared species in the Mediterranean region, and therefore it is subjected to the negative consequences of intensive aquaculture practice such as the occurrence of infectious disease outbreaks. The administration of postbiotics to farmed species represented a sustainable and safe practice with well-known immune-busting properties. This study investigated the secondary effects of butyric acid (commonly used as postbiotic), encapsulated in a starch-based capsule, on sea bream lipid metabolism. Juvenile gilthead seabreams were divided into three experimental groups and fed for 12 weeks three experimental diets: a control group (CTRL) of fish fed the commercial diet, a POP group of fish fed the same commercial diet of the control group added with empty microcapsules and a POP+POST group of fish fed the same commercial diet of the control group added with butyric acid microencapsulated. Analysis of growth parameters (final body weight, FCR, SGR, and daily feed intake) revealed no significant differences among fish from the three experimental groups. Molecular analysis revealed a possible effect of the starch capsule on lipid metabolism. Differently, the administration of POP+POST did not alter the molecular pathways involved in lipid metabolism if compared to the control group.File | Dimensione | Formato | |
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TESI_Sabrin_completa_definitiva1.pdf
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https://hdl.handle.net/20.500.12075/20840