Green algae belonging to the genus Ulva (Chlorophyta) are globally distributed in coastal waters and are capable to grow under a wide variety of conditions, including eutrophic environments. This great adaptability could be exploited in aquaculture activities, where wastewater discharge and eutrophication are often a major issue. The aim of this study was to assess whether Ulva compressa can efficiently grow in wastewater from aquaculture plants. Two strains of U. compressa were sampled from the area of the Passetto di Ancona in the Adriatic Sea. Batch cultures of both strains were grown in triplicate using two growth media: Von Stosch medium (VS) and wastewater from trout grown in a controlled environment in a recirculating system. Photosynthetic activity was characterized in vivo using chlorophyll fluorescence derived parameters (Y(II), ETR(II), NPQ) at the beginning (T0) and at the end (T4) of the experiments. Biomass, nutrient content, protein content and macromolecules ratio (FTIR - Fourier Transform InfraRed spectroscopy) were also analysed. Higher photosynthetic activity, in terms of maximum and effective quantum yields, and biomass increase suggests that specimens grown in the trout wastewater were healthier than those in VS. Protein content observed in U. compressa could be related to the potential luxury uptake due to the high concentrations of nitrogen in both culture mediums. The two different strains showed also a contrasting FTIR profile in terms of carbohydrates. Nonetheless these results are of particular interest and could lay a solid base on which further experiments could be conducted to help guide the development of a more sustainable aquaculture industry.
Le alghe verdi appartenenti al genere Ulva (Chlorophyta) sono specie cosmopolite, tipiche di acque costiere e altamente tolleranti ad un’ampia varietà di condizioni, tra cui gli ambienti eutrofici. Quest’alta capacità di adattamento potrebbe essere sfruttata in acquacoltura dove lo scarico di acque reflue e la conseguente eutrofizzazione sono spesso un problema rilevante. L’obiettivo di questo studio è quello di determinare se Ulva compressa possa crescere in acque reflue da impianti di acquacoltura a terra. Due ceppi di U. compressa sono stati campionati nella zona del Passetto di Ancona (Mar Adriatico). Entrambi sono stati messi in coltura in triplicato utilizzando due mezzi di coltura: Von Stosch (VS) e reflui provenienti da un impianto di allevamento di trote stabulate in ambiente controllato con sistema a ricircolo. L’attività fotosintetica è stata caratterizzata in vivo utilizzando i parametri derivati di fluorescenza della clorofilla (Y(II), ETR(II), NPQ) all’inizio (T0) e alla fine (T4) di ciascun esperimento così come biomassa, concentrazione di nutrienti, contenuto proteico e rapporto tra macromolecole (FTIR – Fourier Transform InfraRed spectroscopy). La maggiore attività fotosintetica, in termini di resa quantica massima e effettiva, e incremento di biomassa osservate suggeriscono che i campioni cresciuti in reflui di trote godano di un migliore stato di salute rispetto e quelli cresciuti all’interno del VS. Il contenuto proteico analizzato in U. compressa ha evidenziato un potenziale luxury uptake dovuto alle grandi quantità di azoto presente in entrambi i mezzi di coltura. I due ceppi inoltre mostrano profili contrastanti in termini di carboidrati negli spettrogrammi FTIR. Questi risultati rivestono comunque una certa rilevanza e formano una solida base dalla quale condurre ulteriori esperimenti per contribuire allo sviluppo di un’acquacoltura più sostenibile.
Valutazione della crescita di Ulva compressa Linnaeus in acque reflue derivanti da impianti di acquacoltura a terra di trota iridea (Oncorhynchus mykiss)
PAUTASSO, STEFANO
2024/2025
Abstract
Green algae belonging to the genus Ulva (Chlorophyta) are globally distributed in coastal waters and are capable to grow under a wide variety of conditions, including eutrophic environments. This great adaptability could be exploited in aquaculture activities, where wastewater discharge and eutrophication are often a major issue. The aim of this study was to assess whether Ulva compressa can efficiently grow in wastewater from aquaculture plants. Two strains of U. compressa were sampled from the area of the Passetto di Ancona in the Adriatic Sea. Batch cultures of both strains were grown in triplicate using two growth media: Von Stosch medium (VS) and wastewater from trout grown in a controlled environment in a recirculating system. Photosynthetic activity was characterized in vivo using chlorophyll fluorescence derived parameters (Y(II), ETR(II), NPQ) at the beginning (T0) and at the end (T4) of the experiments. Biomass, nutrient content, protein content and macromolecules ratio (FTIR - Fourier Transform InfraRed spectroscopy) were also analysed. Higher photosynthetic activity, in terms of maximum and effective quantum yields, and biomass increase suggests that specimens grown in the trout wastewater were healthier than those in VS. Protein content observed in U. compressa could be related to the potential luxury uptake due to the high concentrations of nitrogen in both culture mediums. The two different strains showed also a contrasting FTIR profile in terms of carbohydrates. Nonetheless these results are of particular interest and could lay a solid base on which further experiments could be conducted to help guide the development of a more sustainable aquaculture industry.| File | Dimensione | Formato | |
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https://hdl.handle.net/20.500.12075/25751